Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 70,482 bioRxiv papers from 307,800 authors.

Sensitive red protein calcium indicators for imaging neural activity

By Hod Dana, Boaz Mohar, Yi Sun, Sujatha Narayan, Andrew Gordus, Jeremy P Hasseman, Getahun Tsegaye, Graham T Holt, Amy Hu, Deepika Walpita, Ronak Patel, John J Macklin, Cornelia I. Bargmann, Misha B Ahrens, Eric R. Schreiter, Vivek Jayaraman, Loren L. Looger, Karel Svoboda, Douglas S. Kim

Posted 29 Feb 2016
bioRxiv DOI: 10.1101/041780 (published DOI: 10.7554/eLife.12727)

Genetically encoded calcium indicators (GECIs) allow measurement of activity in large populations of neurons and in small neuronal compartments, over times of milliseconds to months. Although GFP-based GECIs are widely used for in vivo neurophysiology, GECIs with red-shifted excitation and emission spectra have advantages for in vivo imaging because of reduced scattering and absorption in tissue, and a consequent reduction in phototoxicity. However, current red GECIs are inferior to the state-of-the-art GFP-based GCaMP6 indicators for detecting and quantifying neural activity. Here we present improved red GECIs based on mRuby (jRCaMP1a, b) and mApple (jRGECO1a), with sensitivity comparable to GCaMP6. We characterized the performance of the new red GECIs in cultured neurons and in mouse, Drosophila, zebrafish and C. elegans in vivo. Red GECIs facilitate deep-tissue imaging, dual-color imaging together with GFP-based reporters, and the use of optogenetics in combination with calcium imaging.

Download data

  • Downloaded 3,718 times
  • Download rankings, all-time:
    • Site-wide: 829 out of 70,543
    • In neuroscience: 107 out of 12,685
  • Year to date:
    • Site-wide: 31,051 out of 70,543
  • Since beginning of last month:
    • Site-wide: 58,712 out of 70,543

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News