Rxivist logo

Tissue-wide coordination of calcium signaling regulates the epithelial stem cell pool during homeostasis

By Jessica L Moore, Feng Gao, Catherine Matte-Martone, Shuangshuang Du, Elizabeth Lathrop, Smirthy Ganesan, Lin Shao, Dhananjay Bhaskar, Andy Cox, Caroline E Hendry, Bastian A Rieck, Smita Krishnaswamy, Valentina Greco

Posted 13 Oct 2021
bioRxiv DOI: 10.1101/2021.10.12.464066

Ca2+ signaling impacts nearly every aspect of cellular life and must be tightly regulated across tissues to maintain their integrity and function. However, the role of in vivo Ca2+ signaling in non-excitatory mammalian tissues has been largely unexplored due to significant challenges in studying both the spatiotemporal dynamics of the signaling, as well as the cellular complexity and high-dimensionality of data associated with tissue-wide analysis and interpretation. To address this, we implemented live imaging of Ca2+ signaling within the epidermal stem cell layer and developed a computational approach, Geometric Scattering Trajectory Homology (GSTH), to analyze and extract complex signaling patterns. We discover that Ca2+ signaling spreads in a coordinated and directed manner, distinct from signaling in excitatory tissues, and is regulated by connexin 43 (Cx43). Cx43 expression is linked to cell cycle state, peaking in G1, and is essential for proper cell division rates. In contrast to other cell cycle phases, G2 cells are essential for homeostatic patterns of Ca2+ signaling and are essential to maintain the balance of regenerative behaviors in the epidermis. Our model provides a framework to investigate spatiotemporal signaling dynamics in complex tissues, previously not possible.

Download data

  • Downloaded 446 times
  • Download rankings, all-time:
    • Site-wide: 82,903
    • In cell biology: 3,784
  • Year to date:
    • Site-wide: 19,104
  • Since beginning of last month:
    • Site-wide: 1,360

Altmetric data

Distribution of downloads per paper, site-wide