Rxivist logo

Kinetic Explanations for the Sequence Biases Observed in the Nonenzymatic Copying of RNA Templates

By Dian Ding, Lijun Zhou, Constantin Giurgiu, Jack W Szostak

Posted 06 Oct 2021
bioRxiv DOI: 10.1101/2021.10.06.463393

The identification of nonenzymatic pathways for nucleic acid replication is a key challenge in understanding the origin of life. We have previously shown that nonenzymatic RNA primer extension using 2-aminoimidazole (2AI) activated nucleotides occurs primarily through an imidazolium-bridged dinucleotide intermediate. The reactive nature and preorganized structure of the intermediate increase the efficiency of primer extension but remain insufficient to drive extensive copying of RNA templates containing all four canonical nucleotides. To understand the factors that limit RNA copying, we synthesized all ten 2AI-bridged dinucleotide intermediates and measured the kinetics of primer extension in a model. The affinities of the ten dinucleotides for the primer/template/helper complexes vary by over 7,000-fold, consistent with nearest neighbor energetic predictions. Surprisingly, the reaction rates at saturating intermediate concentrations still vary by over 15-fold, with the most weakly binding dinucleotides exhibiting a lower maximal reaction rate. Certain noncanonical nucleotides can decrease sequence dependent differences in affinity and primer extension rate, while monomers bridged to short oligonucleotides exhibit enhanced binding and reaction rates. We suggest that more uniform binding and reactivity of imidazolium-bridged intermediates may lead to the ability to copy arbitrary template sequences under prebiotically plausible conditions.

Download data

  • Downloaded 157 times
  • Download rankings, all-time:
    • Site-wide: 157,611
    • In biochemistry: 4,846
  • Year to date:
    • Site-wide: 40,843
  • Since beginning of last month:
    • Site-wide: 36,839

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

News