Rxivist logo

Standardised method for cardiomyocyte isolation and purification from individual murine neonatal, infant, and adult hearts

By Amy M Nicks, Sara R Holman, Andrea Y Chan, Michael Tsang, Paul E Young, David T Humphreys, Nawazish Naqvi, Ahsan Husain, Ming Li, Nicola J Smith, Siiri E Iismaa, Robert M Graham

Posted 30 Sep 2021
bioRxiv DOI: 10.1101/2021.09.30.462562

Primary cardiomyocytes are invaluable for understanding postnatal heart development. However, a universal method to obtain freshly purified cardiomyocytes without using different age-dependent isolation procedures and cell culture, is lacking. Here, we report the development of a standardised method that allows rapid isolation and purification of high-quality cardiomyocytes from individual neonatal through to adult C57BL/6J murine hearts. Langendorff retrograde perfusion, which is currently limited to adult hearts, was adapted for use in neonatal and infant hearts by developing an easier in situ aortic cannulation technique. Tissue digestion conditions were optimised to achieve efficient digestion of hearts of all ages in a comparable timeframe (<14 min). This resulted in a high yield (1.56-2.2x106 cells/heart) and viability (~70-100%) of cardiomyocytes post-isolation. An immunomagnetic cell separation step was then applied to yield highly purified cardiomyocytes (~95%) as confirmed by immunocytochemistry, flow cytometry, and qRT-PCR. For cell-type specific studies, cardiomyocyte DNA, RNA, and protein could be extracted in sufficient yields to conduct molecular experiments. We generated transcriptomic datasets for neonatal cardiomyocytes from individual hearts, for the first time, which revealed nine sex-specific genes (FDR<0.05) encoded on the sex chromosomes. Finally, we also developed an in situ fixation protocol that preserved the native cytoarchitecture of cardiomyocytes (~94% rod-shaped post-isolation), and used it to evaluate cell morphology during cardiomyocyte maturation, as well as capture spindle-shaped neonatal cells undergoing cytokinesis. Together, these procedures allow molecular and morphological profiling of high-quality cardiomyocytes from individual hearts of any postnatal age.

Download data

  • Downloaded 828 times
  • Download rankings, all-time:
    • Site-wide: 51,278
    • In cell biology: 3,686
  • Year to date:
    • Site-wide: 6,182
  • Since beginning of last month:
    • Site-wide: 4,068

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide