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Fast Assembling of Neuron Fragments in Serial 3D Sections

By Hanbo Chen, Daniel M. Iascone, Nuno Macarico da Costa, Ed S Lein, Tianming Liu, Hanchuan Peng

Posted 29 Dec 2016
bioRxiv DOI: 10.1101/097253 (published DOI: 10.1007/s40708-017-0063-9)

Reconstructing neurons from 3D image-stacks of serial sections of thick brain tissue is very time-consuming and often becomes a bottleneck in high-throughput brain mapping projects. We developed NeuronStitcher, a software suite for stitching non-overlapping neuron fragments reconstructed in serial 3D image sections. With its efficient algorithm and user-friendly interface, NeuronStitcher has been used successfully to reconstruct very large and complex human and mouse neurons.

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