Evaluation of commercially available high-throughput SARS-CoV-2 serological assays for serosurveillance and related applications
By
Mars Stone,
Eduard Grebe,
Hasan Sulaeman,
Clara Di Germanio,
Honey Dave,
Kathleen Kelly,
Brad Biggerstaff,
Brigit O Crews,
Nam Tran,
Keith Jerome,
Thomas N Denny,
Boris Hogema,
Mark Destree,
Jefferson M. Jones,
Natalie J. Thornburg,
Graham Simmons,
Mel Krajden,
Steven Kleinman,
Larry J. Dumont,
Michael Paul Busch
Posted 15 Sep 2021
medRxiv DOI: 10.1101/2021.09.04.21262414
SARS-CoV-2 serosurveys can estimate cumulative incidence for monitoring epidemics but require characterization of employed serological assays performance to inform testing algorithm development and interpretation of results. We conducted a multi-laboratory evaluation of 21 commercial high-throughput SARS-CoV-2 serological assays using blinded panels of 1,000 highly-characterized blood-donor specimens. Assays demonstrated a range of sensitivities (96%-63%), specificities (99%-96%) and precision (IIC 0.55-0.99). Durability of antibody detection in longitudinal samples was dependent on assay format and immunoglobulin target, with anti-spike, direct, or total Ig assays demonstrating more stable, or increasing reactivity over time than anti-nucleocapsid, indirect, or IgG assays. Assays with high sensitivity, specificity and durable antibody detection are ideal for serosurveillance. Less sensitive assays demonstrating waning reactivity are appropriate for other applications, including characterizing antibody responses after infection and vaccination, and detection of anamnestic boosting by reinfections and vaccine breakthrough infections. Assay performance must be evaluated in the context of the intended use.
Download data
- Downloaded 662 times
- Download rankings, all-time:
- Site-wide: 64,679
- In infectious diseases: 4,049
- Year to date:
- Site-wide: 47,140
- Since beginning of last month:
- Site-wide: 76,440
Altmetric data
Downloads over time
Distribution of downloads per paper, site-wide
PanLingua
News
- 27 Nov 2020: The website and API now include results pulled from medRxiv as well as bioRxiv.
- 18 Dec 2019: We're pleased to announce PanLingua, a new tool that enables you to search for machine-translated bioRxiv preprints using more than 100 different languages.
- 21 May 2019: PLOS Biology has published a community page about Rxivist.org and its design.
- 10 May 2019: The paper analyzing the Rxivist dataset has been published at eLife.
- 1 Mar 2019: We now have summary statistics about bioRxiv downloads and submissions.
- 8 Feb 2019: Data from Altmetric is now available on the Rxivist details page for every preprint. Look for the "donut" under the download metrics.
- 30 Jan 2019: preLights has featured the Rxivist preprint and written about our findings.
- 22 Jan 2019: Nature just published an article about Rxivist and our data.
- 13 Jan 2019: The Rxivist preprint is live!