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Fast near-whole brain imaging in adult Drosophila during responses to stimuli and behavior

By Sophie Aimon, Takeo Katsuki, Tongqiu Jia, Logan Grosenick, Michael Broxton, Karl Deisseroth, Terrence J. Sejnowski, Ralph J Greenspan

Posted 10 Dec 2015
bioRxiv DOI: 10.1101/033803 (published DOI: 10.1371/journal.pbio.2006732)

Whole brain recordings give us a global perspective of the brain in action. We describe here a method using light field microscopy to record both whole brain calcium and voltage activity at high speed in behaving adult flies. We show first that global activity maps can be obtained for various stimuli and behaviors. Notably, we found that brain activity increased on a global scale when the fly walked but not when it groomed. Second, maps of spatially distinct sources of activity as well as their time series can be extracted using principal component analysis and independent component analysis. Their characteristic shapes matched the anatomy of sub-neuropil regions and in some cases a specific neuron type. Brain structures that responded to light and odor were consistent with previous reports, confirming the validity of the new technique. We also observed previously uncharacterized behavior-related activity, as well as patterns of spontaneous activity in the central complex.

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