Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 65,255 bioRxiv papers from 289,103 authors.
Temporal proteomic profiling of postnatal human cortical development
Michael S. Breen,
Jordan M. Ramsey,
Michael G. Gottschalk,
Maree J. Webster,
Cynthia Shannon Weickert,
Joseph D. Buxbaum,
Posted 13 Sep 2017
bioRxiv DOI: 10.1101/188565 (published DOI: 10.1038/s41398-018-0306-4)
Posted 13 Sep 2017
Healthy cortical development depends on precise regulation of transcription and translation. However, the relationships between levels of mRNAs and their respective translated proteins have not yet been examined comprehensively in the developing human brain. We surveyed the proteomic landscape of 69 dorsolateral prefrontal cortex samples across seven stages of postnatal life and integrated these data with paired transcriptome data. We detected 911 proteins by liquid chromatography mass-spectrometry, and 83 were significantly associated with postnatal age (FDR P<0.05). Network analysis revealed five modules of co-regulated proteins, three of which correlated with age, including two modules with increasing expression implicated in gliogenesis and NADH-metabolism and one neurogenesis-related module with decreasing expression throughout development. These age-related protein modules overlapped with RNA modules and displayed collinear developmental trajectories between paired RNA and protein expression. We also found that the correspondence between mRNAs and proteins decrease as a function of age, especially for genes involved in neurogenesis and cytoskeleton organization. Finally, intellectual disability and developmental delay genetic risk loci converged on myelination and ATP metabolism-related modules in the proteome and transcriptome, consistent with their neuropathological relevance. Collectively, these findings reveal dynamic aspects of protein regulation in the human prefrontal cortex, providing new insights into development, maturation and disease.
- Downloaded 495 times
- Download rankings, all-time:
- Site-wide: 20,546 out of 65,255
- In neuroscience: 3,439 out of 11,691
- Year to date:
- Site-wide: 34,289 out of 65,255
- Since beginning of last month:
- Site-wide: 46,101 out of 65,255
Downloads over time
Distribution of downloads per paper, site-wide
- Top preprints of 2018
- Paper search
- Author leaderboards
- Overall metrics
- The API
- Email newsletter
- 21 May 2019: PLOS Biology has published a community page about Rxivist.org and its design.
- 10 May 2019: The paper analyzing the Rxivist dataset has been published at eLife.
- 1 Mar 2019: We now have summary statistics about bioRxiv downloads and submissions.
- 8 Feb 2019: Data from Altmetric is now available on the Rxivist details page for every preprint. Look for the "donut" under the download metrics.
- 30 Jan 2019: preLights has featured the Rxivist preprint and written about our findings.
- 22 Jan 2019: Nature just published an article about Rxivist and our data.
- 13 Jan 2019: The Rxivist preprint is live!