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Cross-laboratory analysis of brain cell type transcriptomes with applications to interpretation of bulk tissue data

By Burak Ogan Mancarci, Lilah Toker, Shreejoy J Tripathy, Brenna Li, Brad Rocco, Etienne Sibille, Paul Pavlidis

Posted 22 Nov 2016
bioRxiv DOI: 10.1101/089219 (published DOI: 10.1523/ENEURO.0212-17.2017)

Establishing the molecular diversity of cell types is crucial for the study of the nervous system. We compiled a cross-laboratory database of mouse brain cell type-specific transcriptomes from 36 major cell types from across the mammalian brain using rigorously curated published data from pooled cell type microarray and single cell RNA-sequencing studies. We used these data to identify cell type-specific marker genes, discovering a substantial number of novel markers, many of which we validated using computational and experimental approaches. We further demonstrate that summarized expression of marker gene sets in bulk tissue data can be used to estimate the relative cell type abundance across samples. To facilitate use of this expanding resource, we provide a user-friendly web interface at Neuroexpresso.org.

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