Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 67,588 bioRxiv papers from 298,004 authors.
Structurally conserved primate lncRNAs are transiently expressed during human cortical differentiation and influence cell type specific genes
Andrew R. Field,
Frank M.J. Jacobs,
Ian T. Fiddes,
Alex P.R. Phillips,
Andrea M. Reyes-Ortiz,
Jimi L. Rosenkrantz,
Sofie R. Salama,
Posted 12 Dec 2017
bioRxiv DOI: 10.1101/232553 (published DOI: 10.1016/j.stemcr.2018.12.006)
Posted 12 Dec 2017
The cerebral cortex has expanded in size and complexity in primates, yet the underlying molecular mechanisms are obscure. We generated cortical organoids from human, chimpanzee, orangutan, and rhesus pluripotent stem cells and sequenced their transcriptomes at weekly time points for comparative analysis. We used transcript structure and expression conservation to discover thousands of expressed long non-coding RNAs (lncRNAs). Of 2,975 human, multi-exonic lncRNAs, 2,143 were structurally conserved to chimpanzee, 1,731 to orangutan, and 1,290 to rhesus. 386 human lncRNAs were transiently expressed (TrEx) and a similar expression pattern was often observed in great apes (46%) and rhesus (31%). Many TrEx lncRNAs were associated with neuroepithelium, radial glia, or Cajal-Retzius cells by single cell RNA-sequencing. 3/8 tested by ectopic expression showed ≥2-fold effects on neural genes. This rich resource of primate expression data in early cortical development provides a framework for identifying new, potentially functional lncRNAs.
- Downloaded 856 times
- Download rankings, all-time:
- Site-wide: 9,991 out of 67,516
- In neuroscience: 1,578 out of 12,102
- Year to date:
- Site-wide: 19,861 out of 67,516
- Since beginning of last month:
- Site-wide: 35,851 out of 67,516
Downloads over time
Distribution of downloads per paper, site-wide
- Top preprints of 2018
- Paper search
- Author leaderboards
- Overall metrics
- The API
- Email newsletter
- 21 May 2019: PLOS Biology has published a community page about Rxivist.org and its design.
- 10 May 2019: The paper analyzing the Rxivist dataset has been published at eLife.
- 1 Mar 2019: We now have summary statistics about bioRxiv downloads and submissions.
- 8 Feb 2019: Data from Altmetric is now available on the Rxivist details page for every preprint. Look for the "donut" under the download metrics.
- 30 Jan 2019: preLights has featured the Rxivist preprint and written about our findings.
- 22 Jan 2019: Nature just published an article about Rxivist and our data.
- 13 Jan 2019: The Rxivist preprint is live!