From Structure to Sequence: Identification of polyclonal antibody families using cryoEM
Charles A. Bowman,
Robert N. Kirchdoerfer,
Christopher A Cottrell,
Amit A Upadhyay,
Kimberly M Cirelli,
Diane G. Carnathan,
Chiamaka A. Enemuo,
Leigh M. Sewall,
William R Schief,
Steven E Bosinger,
Andrew B. Ward
Posted 14 Apr 2021
bioRxiv DOI: 10.1101/2021.04.13.439712
Posted 14 Apr 2021
One of the rate-limiting steps in analyzing immune responses to vaccines or infections is the isolation and characterization of monoclonal antibodies. Here, we present a hybrid structural and bioinformatic approach to directly assign the heavy and light chains, identify complementarity- determining regions and discover sequences from cryoEM density maps of serum-derived polyclonal antibodies bound to an antigen. When combined with next generation sequencing of immune repertoires we were able to specifically identify clonal family members, synthesize the monoclonal antibodies and confirm that they interact with the antigen in a manner equivalent to the corresponding polyclonal antibodies. This structure-based approach for identification of monoclonal antibodies from polyclonal sera opens new avenues for analysis of immune responses and iterative vaccine design.
- Downloaded 903 times
- Download rankings, all-time:
- Site-wide: 34,677
- In immunology: 1,155
- Year to date:
- Site-wide: 13,260
- Since beginning of last month:
- Site-wide: 35,296
Downloads over time
Distribution of downloads per paper, site-wide
- 27 Nov 2020: The website and API now include results pulled from medRxiv as well as bioRxiv.
- 18 Dec 2019: We're pleased to announce PanLingua, a new tool that enables you to search for machine-translated bioRxiv preprints using more than 100 different languages.
- 21 May 2019: PLOS Biology has published a community page about Rxivist.org and its design.
- 10 May 2019: The paper analyzing the Rxivist dataset has been published at eLife.
- 1 Mar 2019: We now have summary statistics about bioRxiv downloads and submissions.
- 8 Feb 2019: Data from Altmetric is now available on the Rxivist details page for every preprint. Look for the "donut" under the download metrics.
- 30 Jan 2019: preLights has featured the Rxivist preprint and written about our findings.
- 22 Jan 2019: Nature just published an article about Rxivist and our data.
- 13 Jan 2019: The Rxivist preprint is live!