Rxivist logo

Mechanism of LolCDE as a molecular extruder of bacterial triacylated lipoproteins

By Stuti Sharma, Ruoyu Zhou, Li Wan, Kangkang Song, Chen Xu, Yanyan Li, Maofu Liao

Posted 08 Apr 2021
bioRxiv DOI: 10.1101/2021.04.06.438740

Present in all bacteria, lipoproteins are central in bacterial growth and antibiotic resistance. These proteins use lipid acyl chains attached to the N-terminal cysteine residue to anchor on the outer surface of cytoplasmic membrane. In Gram-negative bacteria, many lipoproteins are transported to the outer membrane (OM), a process dependent on the ATP-binding cassette (ABC) transporter LolCDE which extracts the OM-targeted lipoproteins from the cytoplasmic membrane for subsequent trafficking across the periplasm. Lipid-anchored proteins pose a unique challenge for transport machinery as they have both hydrophobic lipid moieties and soluble protein component, and the underlying mechanism is poorly understood. Here we determined the cryo-EM structures of nanodisc-embedded LolCDE in the nucleotide-free and nucleotide-bound states at 3.8-[A] and 3.5-[A] resolution, respectively. The structural analyses, together with biochemical and mutagenesis studies, uncover how LolCDE specifically recognizes its substrate by establishing multiple interactions with the lipid and N-terminal peptide moieties of the lipoprotein, and identify the amide-linked acyl chain as the key element for LolCDE interaction. Upon nucleotide binding, the transmembrane helices and the periplasmic domains of LolCDE undergo large-scale, asymmetric movements, resulting in extrusion of the captured lipoprotein. Comparison of LolCDE and MacB reveals the conserved mechanism of type VII ABC transporters and emphasizes the unique properties of LolCDE as a molecule extruder of triacylated lipoproteins.

Download data

  • Downloaded 222 times
  • Download rankings, all-time:
    • Site-wide: 109,348
    • In microbiology: 7,983
  • Year to date:
    • Site-wide: 17,884
  • Since beginning of last month:
    • Site-wide: 12,165

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide