Rxivist logo

Redundant mechanisms driven independently by RUNX1 and GATA2 for hematopoietic development

By Erica Bresciani, Blake Carrington, Kai Yu, Erika Kim, Tao Zhen, Victoria Guzman, Elizabeth Broadbridge, Kevin Bishop, Martha Kirby, Ursula Harper, Stephne Wincovitch, Stefania Dell'Orso, Vittorio Sartorelli, Raman Sood, Paul P Liu

Posted 03 Apr 2021
bioRxiv DOI: 10.1101/2021.04.02.438148

RUNX1 is essential for the generation of hematopoietic stem cells (HSCs). Runx1 null mouse embryos lack definitive hematopoiesis and die in mid-gestation. However, even though zebrafish embryos with a runx1 W84X mutation have defects in early definitive hematopoiesis, some runx1W84X/W84X embryos can develop to fertile adults with blood cells of multi-lineages, raising the possibility that HSCs can emerge without RUNX1. Here, using three new zebrafish runx1-/- lines we uncovered the compensatory mechanism for runx1-independent hematopoiesis. We show that, in the absence of a functional runx1, a cd41-GFP+ population of hematopoietic precursors still emerge from the hemogenic endothelium and can colonize the hematopoietic tissues of the mutant embryos. Single-cell RNA sequencing of the cd41-GFP+ cells identified a set of runx1-/--specific signature genes during hematopoiesis. Significantly, gata2b, which normally acts upstream of runx1 for the generation of HSCs, was increased in the cd41-GFP+ cells in runx1- /- embryos. Interestingly, genetic inactivation of both gata2b and its paralog, gata2a, did not affect hematopoiesis. However, knocking out runx1 and any three of the four alleles of gata2a and gata2b abolished definitive hematopoiesis. Gata2 expression was also upregulated in hematopoietic cells in Runx1-/- mice, suggesting the compensatory mechanism is conserved. Our findings indicate that RUNX1 and GATA2 serve redundant roles for HSC production, acting as safeguard for each other.

Download data

  • Downloaded 360 times
  • Download rankings, all-time:
    • Site-wide: 105,191
    • In genetics: 4,298
  • Year to date:
    • Site-wide: 46,713
  • Since beginning of last month:
    • Site-wide: 141,393

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

News