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Interactome analysis of C. elegans synapses by TurboID-based proximity labeling

By Murat Artan, Stephen Barratt, Sean Martin Flynn, Farida Begum, Mark Skehel, Armel Nicolas, Mario de Bono

Posted 01 Apr 2021
bioRxiv DOI: 10.1101/2021.04.01.438103

Proximity labeling provides a powerful in vivo tool to characterize the proteome of sub-cellular structures and the interactome of specific proteins. Using the highly active biotin ligase TurboID, we optimize a proximity labeling protocol for C. elegans. We use this protocol to characterise the proteomes of the worm's gut, muscle, skin, and nervous system. We express TurboID exclusively in the pair of AFD neurons and show we can identify known and previously unknown proteins expressed selectively in AFD. We knock TurboID into the endogenous elks-1 gene, which encodes a presynaptic active zone protein. We identify many known ELKS-1 interacting proteins as well as previously uncharacterised synaptic proteins. Versatile vectors, and the inherent advantage of C. elegans for biochemistry, make proximity labeling a valuable addition to the nematode's armory.

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