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HaloTag9: an engineered protein tag to improve fluorophore performance

By Michelle S. Frei, Miroslaw Tarnawski, Julia Roberti, Birgit Koch, Julien Hiblot, Kai Johnsson

Posted 01 Apr 2021
bioRxiv DOI: 10.1101/2021.04.01.438024

HaloTag9 is an engineered variant of HaloTag7 with up to 40% higher brightness and increased fluorescence lifetime when labeled with fluorogenic rhodamines. Moreover, combining HaloTag9 with HaloTag7 and other fluorescent probes enabled live-cell multiplexing using a single fluorophore and the generation of a fluorescence lifetime-based biosensor. The increased brightness of HaloTag9 and its use in fluorescence lifetime multiplexing makes it a powerful tool for live-cell imaging.

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