Transmembrane kinase family proteins (TMKs) have been implicated in regulating both auxin signaling and plant development. To obtain insights of the potential TMKs' function in plant development, we regenerated new full sets of tmk mutants, and discovered many new phenotypes, such as defective organogenesis, smaller rosette leaves, fertility defects and selfing population defects in different combination of tmk mutants. Taken together, our results demonstrated that TMKs participated in multiple aspects of plant development, which provided a great reference for any future research.
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