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Intra-ocular dendritic cells are increased in HLA-B27 associated acute anterior uveitis

By Maren Kasper, Michael Heming, David Schafflick, Xiaolin Li, Tobias Lautwein, Melissa Meyer zu Horste, Dirk Bauer, Karoline Walscheid, Heinz Wiendl, Karin Loser, Arnd Heiligenhaus, Gerd Meyer zu Hörste

Posted 18 Feb 2021
bioRxiv DOI: 10.1101/2021.02.16.431370

Uveitis describes a heterogeneous group of inflammatory eye diseases characterized by infiltration of leukocytes into the uveal tissues. HLA-B27-associated Uveitis is a common subtype of uveitis and a prototypical ocular autoimmune disease. Local immune mechanisms driving human uveitis are poorly characterized mainly due to the limited available biomaterial and subsequent technical limitations. Here, we provide the first high-resolution characterization of intraocular leukocytes in HLA-B27 positive and negative AU and an infectious endophthalmitis control by combining single cell RNA sequencing (scRNA-seq) with flow cytometry and low-input proteomics. Ocular cell infiltrates consisted primarily of lymphocytes in uveitis and of myeloid cells in infectious endophthalmitis. HLA-B27 positive uveitis exclusively featured an plasmacytoid and classical dendritic cells (cDC) infiltrate, and of plasma cells. Moreover, cDCs were central in predicted local cell-cell communication. The data suggests a unique pattern of ocular leukocyte infiltration in HLA-B27 positive uveitis with relevance of particular dendritic cells.

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