Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 57,313 bioRxiv papers from 263,927 authors.

h-channels contribute to divergent electrophysiological properties of supragranular pyramidal neurons in human versus mouse cerebral cortex

By Brian Kalmbach, Anatoly Buchin, Jeremy A Miller, Trygve E Bakken, Rebecca D Hodge, Peter Chong, Rebecca de Frates, Kael Dai, Ryder P Gwinn, Charles Cobbs, Andrew L Ko, Jeffrey G Ojemann, Daniel L Silbergeld, Christof Koch, Costas A Anastassiou, Ed Lein, Jonathan T Ting

Posted 02 May 2018
bioRxiv DOI: 10.1101/312298

Gene expression studies suggest that differential ion channel expression contributes to differences in rodent versus human neuronal physiology. We tested whether h-channels more prominently contribute to the physiological properties of human compared to mouse supragranular pyramidal neurons. Single cell/nucleus RNA sequencing revealed ubiquitous HCN1-subunit expression in excitatory neurons in human, but not mouse supragranular layers. Using patch-clamp recordings, we found stronger h-channel-related membrane properties in supragranular pyramidal neurons in human temporal cortex, compared to mouse supragranular pyramidal neurons in temporal association area. The magnitude of these differences depended upon cortical depth and was largest in pyramidal neurons in deep L3. Additionally, pharmacologically blocking h-channels produced a larger change in membrane properties in human compared to mouse neurons. Finally, using biophysical modeling, we provided evidence that h-channels promote the transfer of theta frequencies from dendrite-to-soma in human L3 pyramidal neurons. Thus, h-channels contribute to between-species differences in a fundamental neuronal property.

Download data

  • Downloaded 611 times
  • Download rankings, all-time:
    • Site-wide: 13,374 out of 57,313
    • In neuroscience: 2,181 out of 10,055
  • Year to date:
    • Site-wide: 33,718 out of 57,313
  • Since beginning of last month:
    • Site-wide: 23,040 out of 57,313

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News