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The total mRNA concentration buffering system in yeast is global rather than gene-specific

By Jose Garcia-Martinez, Daniel A Medina, Pablo Bellvis, Mai Sun, Patrick Cramer, Sebastian Chavez, Jose E. Perez-Ortin

Posted 16 Jan 2021
bioRxiv DOI: 10.1101/2021.01.14.426689

Gene expression in eukaryotes does not follow a linear process from transcription to translation and mRNA degradation. Instead it follows a circular process in which cytoplasmic mRNA decay crosstalks with nuclear transcription. In many instances this crosstalk contributes to buffer mRNA at a roughly constant concentration. Whether the mRNA buffering concept operates on the total mRNA concentration or at the gene-specific level, and if the mechanism to do so is a global or a specific one, remain unknown. Here we assessed changes in mRNA concentrations and their synthesis rates (SRs) along the transcriptome of aneuploid strains of the yeast Saccharomyces cerevisiae. We also assessed mRNA concentrations and their SRs in non sense-mediated decay (NMD) targets in euploid strains. We found that the altered SRs in the genes from the aneuploid chromosome and the changes in their mRNA stabilities were not balanced. In addition, the stability of NMD targets was not specifically counterbalanced by the changes in SR. We conclude that there is no genetic compensation of NMD mRNA targets, and total mRNA buffering uses mostly a global system rather than a gene-specific one in yeast.

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