Rxivist logo

In vivo structure and dynamics of the RNA genome of SARS-Cov-2

By Yan Zhang, Kun C. Huang, Dejian Xie, Jian You Lau, Wenlong Shen, Ping Li, Dong Wang, Zhong Zou, Shu Shi, Hongguang Ren, Meilin Jin, Grzegorz Kudla, Zhihu Zhao

Posted 15 Jan 2021
bioRxiv DOI: 10.1101/2021.01.15.426526

The SARS-CoV-2 coronavirus, which causes the COVID-19 pandemic, is one of the largest positive strand RNA viruses. Here we developed a simplified SPLASH assay and comprehensively mapped the in vivo RNA-RNA interactome of SARS-CoV-2 RNA during the viral life cycle. We observed canonical and alternative structures including 3-UTR and 5-UTR, frameshifting element (FSE) pseudoknot and genome cyclization in cells and in virions. We provide direct evidence of interactions between Transcription Regulating Sequences (TRS-L and TRS-Bs), which facilitate discontinuous transcription. In addition, we reveal alternative short and long distance arches around FSE, forming a "high-order pseudoknot" embedding FSE, which might help ribosome stalling at frameshift sites. More importantly, we found that within virions, while SARS-CoV-2 genome RNA undergoes intensive compaction, genome cyclization is weakened and genome domains remain stable. Our data provides a structural basis for the regulation of replication, discontinuous transcription and translational frameshifting, describes dynamics of RNA structures during life cycle of SARS-CoV-2, and will help to develop antiviral strategies.

Download data

  • Downloaded 703 times
  • Download rankings, all-time:
    • Site-wide: 37,132
    • In genomics: 3,138
  • Year to date:
    • Site-wide: 3,379
  • Since beginning of last month:
    • Site-wide: 31,630

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

News