Cas9-cleavage sequences in minimal plasmids enhance non-viral genome targeting of CARs in primary human T cells
Posted 02 Jan 2021
bioRxiv DOI: 10.1101/2020.12.31.424920
Posted 02 Jan 2021
T cell genome editing holds great promise to advance a range of immunotherapies but is encumbered by the dependence on difficult-to-produce and expensive viral vectors. Here we have designed small double-stranded plasmid DNA modified to mediate high-efficiency homologous recombination. The resulting chimeric antigen receptor (CAR)-T cells display a similar phenotype, transcriptional profile and in vivo potency as CAR-T cells generated using adeno-associated viral (AAV) vector. This method should simplify and accelerate the use of precision engineering to produce edited T cells for research and clinical purposes.
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