Rxivist logo

An efficient lentiviral CRISPRi approach to silence genes in primary human monocytes

By Bingxu Liu, Matteo Gentili, David Lieb, Thomas Eisenhaure, Darrell J Irvine, Nir Hacohen

Posted 24 Dec 2020
bioRxiv DOI: 10.1101/2020.12.23.424242

Human primary monocytes are critical in controlling immune responses and tissue homeostasis. However, to identify and study molecular components that underlie the function of these cells, there remains a need to develop methods to perturb genes in these cells. Here we report lentiviral-based delivery of dCas9-KRAB and guide RNAs to efficiently silent target genes, such as CD45 and CD209. We further show that sgRNAs against TICAM1 dampen proinflammatory cytokine and interferon expression in response to lipopolysaccharide. When delivered by lentivirus, sgRNAs are incorporated into the genome, thus enabling pooled screening to perturb and identify coding and non-coding elements that contribute to the functions of primary human monocytes.

Download data

  • Downloaded 232 times
  • Download rankings, all-time:
    • Site-wide: 99,925
    • In immunology: 2,841
  • Year to date:
    • Site-wide: 19,458
  • Since beginning of last month:
    • Site-wide: 45,977

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News