Classification of electrophysiological and morphological types in mouse visual cortex
Nathan W. Gouwens,
Staci A. Sorensen,
Susan M. Sunkin,
L. Daigle Tanya,
Rebecca A de Frates,
Thuc Nghi Nguyen,
Brian R. Lee,
Gabe J. Murphy,
Philip R. Nicovich,
Michael J. Hawrylycz,
John W. Phillips,
Posted 17 Jul 2018
bioRxiv DOI: 10.1101/368456 (published DOI: 10.1038/s41593-019-0417-0)
Posted 17 Jul 2018
Understanding the diversity of cell types in the brain has been an enduring challenge and requires detailed characterization of individual neurons in multiple dimensions. To profile morpho-electric properties of mammalian neurons systematically, we established a single cell characterization pipeline using standardized patch clamp recordings in brain slices and biocytin-based neuronal reconstructions. We built a publicly-accessible online database, the Allen Cell Types Database, to display these data sets. Intrinsic physiological and morphological properties were measured from over 1,800 neurons from the adult laboratory mouse visual cortex. Quantitative features were used to classify neurons into distinct types using unsupervised methods. We establish a taxonomy of morphologically- and electrophysiologically-defined cell types for this region of cortex with 17 e-types and 35 m-types, as well as an initial correspondence with previously-defined transcriptomic cell types using the same transgenic mouse lines.
- Downloaded 4,366 times
- Download rankings, all-time:
- Site-wide: 1,002 out of 93,208
- In neuroscience: 103 out of 16,589
- Year to date:
- Site-wide: 4,900 out of 93,208
- Since beginning of last month:
- Site-wide: 4,768 out of 93,208
Downloads over time
Distribution of downloads per paper, site-wide
- 18 Dec 2019: We're pleased to announce PanLingua, a new tool that enables you to search for machine-translated bioRxiv preprints using more than 100 different languages.
- 21 May 2019: PLOS Biology has published a community page about Rxivist.org and its design.
- 10 May 2019: The paper analyzing the Rxivist dataset has been published at eLife.
- 1 Mar 2019: We now have summary statistics about bioRxiv downloads and submissions.
- 8 Feb 2019: Data from Altmetric is now available on the Rxivist details page for every preprint. Look for the "donut" under the download metrics.
- 30 Jan 2019: preLights has featured the Rxivist preprint and written about our findings.
- 22 Jan 2019: Nature just published an article about Rxivist and our data.
- 13 Jan 2019: The Rxivist preprint is live!