Rxivist logo

Post-translational thioamidation of methyl-coenzyme M reductase, a key enzyme in methanogenic and methanotrophic Archaea

By Dipti D. Nayak, Nilkamal Mahanta, Douglas A Mitchell, William W. Metcalf

Posted 27 Mar 2017
bioRxiv DOI: 10.1101/121111 (published DOI: 10.7554/eLife.29218)

The enzyme methyl-coenzyme M reductase (MCR), found in strictly anaerobic methanogenic and methanotrophic archaea, catalyzes a reversible reaction involved in the production and consumption of the potent greenhouse gas methane. The α subunit of this enzyme (McrA) contains several unusual post-translational modifications, including an exceptionally rare thioamidation of glycine. Based on the presumed function of homologous genes involved in the biosynthesis of thioamide-containing natural products, we hypothesized that the archaeal tfuA and ycaO genes would be responsible for post-translational installation of thioglycine into McrA. Mass spectrometric characterization of McrA in a ΔycaO-tfuA mutant of the methanogenic archaeon Methanosarcina acetivorans revealed the presence of glycine, rather than thioglycine, supporting this hypothesis. Physiological characterization of this mutant suggested a new role for the thioglycine modification in enhancing protein stability, as opposed to playing a direct catalytic role. The universal conservation of this modification suggests that MCR arose in a thermophilic ancestor.

Download data

  • Downloaded 775 times
  • Download rankings, all-time:
    • Site-wide: 49,484
    • In biochemistry: 1,198
  • Year to date:
    • Site-wide: 126,620
  • Since beginning of last month:
    • Site-wide: 104,180

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

News