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ReScan, a Multiplex Diagnostic Pipeline, Pans Human Sera for SARS-CoV-2 Antigens

By Colin R. Zamecnik, Jayant V. Rajan, Kevin A Yamauchi, Sabrina A Mann, Gavin M. Sowa, Kelsey Zorn, Bonny D. Alvarenga, Mars Stone, Philip J. Norris, Wei Gu, Charles Y Chiu, Joseph L. DeRisi, Michael R Wilson

Posted 13 May 2020
medRxiv DOI: 10.1101/2020.05.11.20092528

Serologic assays are needed to determine SARS-CoV-2 seroprevalence, but poor specificity can overestimate exposures. Here, we built a pan-human coronavirus proteome-wide programmable phage display assay (VirScan) to profile coronavirus antigens specifically enriched by 20 COVID-19 patient serum IgG. With ReScan, a new diagnostic development workflow which combines the isolation of phage expressing the most immunogenic peptides with paper-based microarrays manufactured via acoustic liquid handling, we identified 9 candidate antigens from a library of 534 SARS-CoV-2 peptides. These arrays could form the basis of a multiplexed COVID-19 serologic assay with enhanced specificity. ReScan has broad applicability for serologic assay development.

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