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Regulation by HSP70/90 in the different tissues and testis development of male cattle (Cattle-yak and Yak)

By Penggang Liu, Sijiu Yu, Yan Cui, Junfeng He, Qian Zhang, Jun Liu, Liangli Song, Yuanfang Xu, Ting Wang, Shengnan Zou, Hui Li

Posted 16 Aug 2018
bioRxiv DOI: 10.1101/393371

HSP7090 play important role in testis develop and spermatozoa regulation, but the contact of HSP7090 with infertility in cattle is unclear. Here, we focus on male cattleyak and yak, which to investigate the expression and localization of HSP7090 in different tissues, and explore the influence of HSP7090 to infertility. In our study, a total of 54 cattle 24 cattleyak and 30 yak were examined. The HSP90 mRNA of cattleyak was cloned first and found amino acid variation in HSP90, which led to difference at protein spatial structure compare with yak. To investigate whether the expression of HSP7090 mRNA and protein are different in cattleyak and yak, we used realtime quantitative PCR and Western blot to examine them. We found that the expression level of HSP7090 mRNA and protein are disparity in different tissues and testis development stages, and obviously high expression was observed in testicle during juvenile and adult, Moreover, its interestingly in which the HSP70 expression is significant high in yak whereas HSP90 in cattleyak. On this bases, we detect the location of HSP7090 in testis by immunohistochemical and immunofluorescence, the results demonstrate that HSP7090 were located in the epithelial cells, spermatogenic cells and mesenchymal cells. In summary, our study proved the expression of HSP7090 are different in tissues, and the expression of HSP90 is obviously high in testis of cattleyak, which propose that the infertility of cattleyak may cause from upregulating of HSP90.

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