Activated Expression of Master Regulator MYB31 and of Capsaicinoid Biosynthesis Genes Results in Capsaicinoid Biosynthesis and Accumulation in the Pericarp of the Extremely Pungent Capsicum chinense
Posted 05 Nov 2020
bioRxiv DOI: 10.1101/2020.11.05.369454
Posted 05 Nov 2020
Capsaicinoids confer pungency in Capsicum fruits, and the capsaicinoid content varies greatly among the five domesticated Capsicum species. Although it is generally recognized that capsaicinoid biosynthesis occurs exclusively in the placenta, few studies have focused on capsaicinoid biosynthesis gene (CBG) expression in the pericarp. Therefore, the transcriptional regulation mechanisms of capsaicinoid biosynthesis in the pericarp remain elusive. Here, the capsaicinoid contents of 32 accessions from five domesticated Capsicum species were analyzed. The results showed that the capsaicinoid contents of C. chinense accessions are significantly higher than those of the other four Capsicum species due to the increased accumulation of capsaicinoids, especially in the pericarp. Compared to that in accessions with low pericarp capsaicinoid content, the expression of the master regulator MYB31 is significantly upregulated in the pericarp in C. chinense accessions, which leads to high levels of CBG expression. Moreover, in fruits of the extremely pungent Trinidad Moruga Scorpion (C. chinense) and low-pungency 59 inbred line (C. annuum) at different developmental stages, the capsaicinoid accumulation patterns were consistent with the MYB31 and CBG expression levels in the pericarp. Taken together, our results provide novel insights into the molecular mechanism arising from the expression of a master regulator in the pericarp that results in exceedingly hot peppers. The genetic resources identified in this study could be used as genetic resources for the genetic improvement of pepper pungency. ### Competing Interest Statement The authors have declared no competing interest.
- Downloaded 306 times
- Download rankings, all-time:
- Site-wide: 158,509
- In plant biology: 4,387
- Year to date:
- Site-wide: 76,017
- Since beginning of last month:
- Site-wide: 54,331
Downloads over time
Distribution of downloads per paper, site-wide
- 27 Nov 2020: The website and API now include results pulled from medRxiv as well as bioRxiv.
- 18 Dec 2019: We're pleased to announce PanLingua, a new tool that enables you to search for machine-translated bioRxiv preprints using more than 100 different languages.
- 21 May 2019: PLOS Biology has published a community page about Rxivist.org and its design.
- 10 May 2019: The paper analyzing the Rxivist dataset has been published at eLife.
- 1 Mar 2019: We now have summary statistics about bioRxiv downloads and submissions.
- 8 Feb 2019: Data from Altmetric is now available on the Rxivist details page for every preprint. Look for the "donut" under the download metrics.
- 30 Jan 2019: preLights has featured the Rxivist preprint and written about our findings.
- 22 Jan 2019: Nature just published an article about Rxivist and our data.
- 13 Jan 2019: The Rxivist preprint is live!