Rxivist logo

Waffle Method: A general and flexible approach for FIB-milling small and anisotropically oriented samples

By Kotaro Kelley, Ashleigh M. Raczkowski, Oleg Klykov, Pattana Jaroenlak, Daija Bobe, Mykhailo Kopylov, Edward T Eng, Gira Bhabha, Clinton S. Potter, Bridget Carragher, Alex J. Noble

Posted 28 Oct 2020
bioRxiv DOI: 10.1101/2020.10.28.359372

Cryo-FIB/SEM combined with cryo-ET has emerged from within the field of cryo-EM as the method for obtaining the highest resolution structural information of complex biological samples in-situ in native and non-native environments. However, challenges remain in conventional cryo-FIB/SEM workflows, including milling thick specimens that do not vitrify well, specimens with preferred orientation, low-throughput when milling small and/or low concentration specimens, and cellular specimens that distribute poorly across grid squares. Here we present a general approach we call the 'Waffle Method' which leverages high-pressure freezing to address these challenges. We illustrate the mitigation of these challenges by applying the Waffle Method and cryo-ET to reveal the macrostructure of the polar tube in microsporidian spores in multiple complementary orientations, which was previously not possible due to preferred orientation of the spores on the grid. We demonstrate the broadness of the Waffle Method by applying it to three additional cellular samples and a single particle sample using a variety of cryo-FIB-milling hardware, with both manual and automated approaches. We also present a unique and critical stress-relief gap designed specifically for waffled lamellae. Additionally, we describe applications of the Waffle Method which are currently being explored. We propose the Waffle Method as a way to achieve many of the advantages of cryo-liftout on the specimen grid while avoiding the long, challenging, and technically-demanding process required for cryo-liftout.

Download data

  • Downloaded 2,526 times
  • Download rankings, all-time:
    • Site-wide: 7,067
    • In microbiology: 423
  • Year to date:
    • Site-wide: 2,566
  • Since beginning of last month:
    • Site-wide: 8,966

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

News