Author: Feng Zhang

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Preprints

Protocol: Genome-scale CRISPR-Cas9 Knockout and Transcriptional Activation Screening

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  • Site-wide: 223
  • In molecular biology: 2
• Year to date:
  • Site-wide: 4,295
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  • Site-wide: 4,295

LAMP-Seq: Population-Scale COVID-19 Diagnostics Using Combinatorial Barcoding

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  • Site-wide: 390
  • In molecular biology: 6
• Year to date:
  • Site-wide: 794
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  • Site-wide: 794

Improved vectors and genome-wide libraries for CRISPR screening

• Downloaded 10,744 times
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  • Site-wide: 681
  • In genomics: 55
• Year to date:
  • Site-wide: 14,851
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  • Site-wide: 14,851

DNA microscopy: Optics-free spatio-genetic imaging by a stand-alone chemical reaction

• Downloaded 9,273 times
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  • Site-wide: 822
  • In bioengineering: 7
• Year to date:
  • Site-wide: 38,621
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  • Site-wide: 38,621

Pooled optical screens in human cells

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  • Site-wide: 849
  • In genomics: 72
• Year to date:
  • Site-wide: 6,229
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  • Site-wide: 6,229

DroNc-Seq: Deciphering cell types in human archived brain tissues by massively-parallel single nucleus RNA-seq

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  • Site-wide: 1,187
  • In genomics: 116
• Year to date:
  • Site-wide: 39,017
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  • Site-wide: 39,017

CRISPR/Cas9-APEX-mediated proximity labeling enables discovery of proteins associated with a predefined genomic locus in living cells

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  • Site-wide: 1,340
  • In biochemistry: 17
• Year to date:
  • Site-wide: 28,229
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  • Site-wide: 28,229

In vivo Perturb-Seq reveals neuronal and glial abnormalities associated with Autism risk genes

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  • Site-wide: 1,406
  • In genomics: 135
• Year to date:
  • Site-wide: 2,703
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  • Site-wide: 2,703

Genomic determinants of pathogenicity in SARS-CoV-2 and other human coronaviruses

• Downloaded 5,854 times
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  • Site-wide: 1,488
  • In microbiology: 123
• Year to date:
  • Site-wide: 25,991
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  • Site-wide: 25,991

C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector

• Downloaded 4,737 times
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  • Site-wide: 2,002
  • In molecular biology: 38
• Year to date:
  • Site-wide: 68,727
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  • Site-wide: 68,727

Multiplex gene editing by CRISPR-Cpf1 through autonomous processing of a single crRNA array

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  • Site-wide: 2,014
  • In molecular biology: 41
• Year to date:
  • Site-wide: 34,317
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  • Site-wide: 34,317

High-resolution interrogation of functional elements in the noncoding genome

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  • Site-wide: 2,511
  • In molecular biology: 53
• Year to date:
  • Site-wide: 60,333
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  • Site-wide: 60,333

A cellular and spatial map of the choroid plexus across brain ventricles and ages

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  • Site-wide: 2,811
  • In neuroscience: 211
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  • Site-wide: None
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Div-Seq: A single nucleus RNA-Seq method reveals dynamics of rare adult newborn neurons in the CNS

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  • Site-wide: 2,843
  • In neuroscience: 214
• Year to date:
  • Site-wide: 51,107
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Genome Editing With Targeted Deaminases

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  • Site-wide: 2,902
  • In bioengineering: 28
• Year to date:
  • Site-wide: 47,105
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  • Site-wide: 47,105

A Survey of Genome Editing Activity for 16 Cpf1 orthologs

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  • Site-wide: 3,790
  • In molecular biology: 86
• Year to date:
  • Site-wide: 54,322
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  • Site-wide: 54,322

Integrated design, execution, and analysis of arrayed and pooled CRISPR genome editing experiments

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  • Site-wide: 4,012
  • In molecular biology: 90
• Year to date:
  • Site-wide: 38,923
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  • Site-wide: 38,923

Engineered Cpf1 Enzymes with Altered PAM Specificities

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  • Site-wide: 6,965
  • In synthetic biology: 76
• Year to date:
  • Site-wide: 110,602
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  • Site-wide: 110,602

BLISS: quantitative and versatile genome-wide profiling of DNA breaks in situ

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  • Site-wide: 9,645
  • In molecular biology: 258
• Year to date:
  • Site-wide: 57,158
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  • Site-wide: 57,158

Direct in vivo mapping of functional suppressors in glioblastoma genome

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  • Site-wide: 10,267
  • In systems biology: 194
• Year to date:
  • Site-wide: 42,799
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  • Site-wide: 42,799

Cas13b is a Type VI-B CRISPR-associated RNA-Guided RNase differentially regulated by accessory proteins Csx27 and Csx28

• Downloaded 1,539 times
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  • Site-wide: 10,300
  • In microbiology: 564
• Year to date:
  • Site-wide: 109,082
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  • Site-wide: 109,082

The CAFA challenge reports improved protein function prediction and new functional annotations for hundreds of genes through experimental screens

• Downloaded 1,506 times
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  • Site-wide: 10,638
  • In bioinformatics: 1,311
• Year to date:
  • Site-wide: 24,541
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  • Site-wide: 24,541

Pooled AAV-CRISPR Screen with Targeted Amplicon Sequencing

• Downloaded 1,350 times
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  • Site-wide: 12,499
  • In systems biology: 249
• Year to date:
  • Site-wide: 88,750
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  • Site-wide: 88,750

Human Pluripotent Stem Cell-Derived Neural Cells and Brain Organoids Reveal SARS-CoV-2 Neurotropism

• Downloaded 1,261 times
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  • Site-wide: 13,851
  • In neuroscience: 1,662
• Year to date:
  • Site-wide: 11,080
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  • Site-wide: 11,080

GUIDES: sgRNA design for loss-of-function screens

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  • Site-wide: 14,870
  • In genetics: 763
• Year to date:
  • Site-wide: 97,698
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  • Site-wide: 97,698

Assessing the impact of a symptom-based mass screening and testing intervention during a novel infectious disease outbreak: The case of COVID-19

• Downloaded 956 times
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  • Site-wide: 20,931
  • In infectious diseases: 1,613
• Year to date:
  • Site-wide: 46,347
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  • Site-wide: 46,347

Ongoing Global and Regional Adaptive Evolution of SARS-CoV-2

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  • Site-wide: 24,226
  • In microbiology: 1,380
• Year to date:
  • Site-wide: 6,670
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  • Site-wide: 6,670

The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterflies

• Downloaded 651 times
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  • Site-wide: 35,718
  • In evolutionary biology: 1,853
• Year to date:
  • Site-wide: 17,536
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  • Site-wide: 17,536

Optimization of multiplexed CRISPR/Cas9 system for highly efficient genome editing in Setaria viridis

• Downloaded 640 times
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  • Site-wide: 36,531
  • In plant biology: 771
• Year to date:
  • Site-wide: 29,212
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  • Site-wide: 29,212

Essentiality of CREBBP in EP300 truncated B-cell lymphoma revealed by genome-wide CRISPR-Cas9 screen

• Downloaded 625 times
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  • Site-wide: 37,719
  • In cancer biology: 1,021
• Year to date:
  • Site-wide: 17,695
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  • Site-wide: 17,695

Novel gene-specific translation mechanism of dysregulated, chronic inflammation reveals promising, multifaceted COVID-19 therapeutics

• Downloaded 560 times
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  • Site-wide: 43,246
  • In systems biology: 1,010
• Year to date:
  • Site-wide: 7,896
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  • Site-wide: 7,896

An R Implementation of Tumor-Stroma-Immune Transcriptome Deconvolution Pipeline using DeMixT

• Downloaded 521 times
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  • Site-wide: 47,236
  • In bioinformatics: 4,970
• Year to date:
  • Site-wide: 70,095
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  • Site-wide: 70,095

Multi-cellular communities are perturbed in the aging human brain and with Alzheimer's disease

• Downloaded 513 times
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  • Site-wide: 48,102
  • In neuroscience: 7,097
• Year to date:
  • Site-wide: 1,771
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  • Site-wide: 1,771

A Genetically Encoded Trimethylsilyl 1D 1H-NMR Probe for Conformation Change in Large Membrane Protein Complexes

• Downloaded 442 times
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  • Site-wide: 56,802
  • In biochemistry: 1,505
• Year to date:
  • Site-wide: 63,972
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  • Site-wide: 63,972

PRH1 mediates ARF7-LBD dependent auxin signaling to regulate lateral root development in Arabidopsis thaliana

• Downloaded 442 times
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  • Site-wide: 56,701
  • In plant biology: 1,428
• Year to date:
  • Site-wide: 97,949
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  • Site-wide: 97,949

Movement behavioral plasticity of benthic diatoms driven by optimal foraging

• Downloaded 403 times
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  • Site-wide: 62,420
  • In ecology: 1,765
• Year to date:
  • Site-wide: 48,148
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  • Site-wide: 48,148

Conserved allosteric pathways for activation of TRPV3 revealed through engineering vanilloid-sensitivity

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  • Site-wide: 72,722
  • In neuroscience: 11,197
• Year to date:
  • Site-wide: 65,478
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  • Site-wide: 65,478

Regulatory B cells contribute to the alleviation of colitis induced by Dextran Sulphate Sodium after H.pylori infection

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  • Site-wide: 79,372
  • In immunology: 2,155
• Year to date:
  • Site-wide: 66,672
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  • Site-wide: 66,672

Effect of recombinant phycocyanin on photosynthetic system in Chlamydomonas reinhardtii

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  • Site-wide: 99,252
  • In molecular biology: 2,967
• Year to date:
  • Site-wide: 50,440
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  • Site-wide: 50,440

Photosynthetic resistance and resilience under drought and rewatering in maize plants

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  • Site-wide: 115,489
  • In plant biology: 3,215
• Year to date:
  • Site-wide: 85,807
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  • Site-wide: 85,807

Regulating AMPK and insulin level by Vinegar, Swimming and Refeeding on High-Fat Diet Rats to Rebuild Lipid Homeostasis

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  • Site-wide: 115,569
  • In molecular biology: 3,483
• Year to date:
  • Site-wide: 113,203
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  • Site-wide: 113,203