Author: Feng Zhang

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Preprints

Protocol: Genome-scale CRISPR-Cas9 Knockout and Transcriptional Activation Screening

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  • Site-wide: 237
  • In molecular biology: 3
• Year to date:
  • Site-wide: 1,618
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  • Site-wide: 1,743

LAMP-Seq: Population-Scale COVID-19 Diagnostics Using Combinatorial Barcoding

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  • Site-wide: 402
  • In molecular biology: 7
• Year to date:
  • Site-wide: 1,050
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  • Site-wide: 1,493

Improved vectors and genome-wide libraries for CRISPR screening

• Downloaded 11,023 times
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  • Site-wide: 727
  • In genomics: 53
• Year to date:
  • Site-wide: 6,336
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  • Site-wide: 5,021

DNA microscopy: Optics-free spatio-genetic imaging by a stand-alone chemical reaction

• Downloaded 9,298 times
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  • Site-wide: 906
  • In bioengineering: 7
• Year to date:
  • Site-wide: 54,623
• Since beginning of last month:
  • Site-wide: 56,101

Pooled optical screens in human cells

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  • Site-wide: 929
  • In genomics: 72
• Year to date:
  • Site-wide: 9,981
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  • Site-wide: 12,957

DroNc-Seq: Deciphering cell types in human archived brain tissues by massively-parallel single nucleus RNA-seq

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  • Site-wide: 1,296
  • In genomics: 117
• Year to date:
  • Site-wide: 42,766
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  • Site-wide: 27,969

In vivo Perturb-Seq reveals neuronal and glial abnormalities associated with Autism risk genes

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  • Site-wide: 1,470
  • In genomics: 132
• Year to date:
  • Site-wide: 3,377
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  • Site-wide: 4,040

CRISPR/Cas9-APEX-mediated proximity labeling enables discovery of proteins associated with a predefined genomic locus in living cells

• Downloaded 6,349 times
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  • Site-wide: 1,474
  • In biochemistry: 19
• Year to date:
  • Site-wide: 39,241
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  • Site-wide: 40,879

Genomic determinants of pathogenicity in SARS-CoV-2 and other human coronaviruses

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  • Site-wide: 1,596
  • In microbiology: 133
• Year to date:
  • Site-wide: 16,978
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  • Site-wide: 19,721

A cellular and spatial map of the choroid plexus across brain ventricles and ages

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  • Site-wide: 2,195
  • In neuroscience: 134
• Year to date:
  • Site-wide: 1,312
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  • Site-wide: 1,540

C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector

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  • Site-wide: 2,209
  • In molecular biology: 44
• Year to date:
  • Site-wide: 74,299
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  • Site-wide: 77,824

Multiplex gene editing by CRISPR-Cpf1 through autonomous processing of a single crRNA array

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  • Site-wide: 2,224
  • In molecular biology: 45
• Year to date:
  • Site-wide: 52,091
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  • Site-wide: 71,638

High-resolution interrogation of functional elements in the noncoding genome

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  • Site-wide: 2,776
  • In molecular biology: 57
• Year to date:
  • Site-wide: 73,538
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  • Site-wide: 54,720

Div-Seq: A single nucleus RNA-Seq method reveals dynamics of rare adult newborn neurons in the CNS

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  • Site-wide: 3,086
  • In neuroscience: 226
• Year to date:
  • Site-wide: 44,593
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  • Site-wide: 87,411

Genome Editing With Targeted Deaminases

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  • Site-wide: 3,189
  • In bioengineering: 34
• Year to date:
  • Site-wide: 65,909
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  • Site-wide: 76,550

A Survey of Genome Editing Activity for 16 Cpf1 orthologs

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  • Site-wide: 4,072
  • In molecular biology: 89
• Year to date:
  • Site-wide: 38,481
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  • Site-wide: 31,927

Integrated design, execution, and analysis of arrayed and pooled CRISPR genome editing experiments

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  • Site-wide: 4,317
  • In molecular biology: 96
• Year to date:
  • Site-wide: 35,882
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  • Site-wide: 34,326

Engineered Cpf1 Enzymes with Altered PAM Specificities

• Downloaded 2,012 times
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  • Site-wide: 7,603
  • In synthetic biology: 78
• Year to date:
  • Site-wide: 91,780
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  • Site-wide: 48,344

Ongoing Global and Regional Adaptive Evolution of SARS-CoV-2

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  • Site-wide: 8,025
  • In microbiology: 457
• Year to date:
  • Site-wide: 1,087
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  • Site-wide: 6,086

BLISS: quantitative and versatile genome-wide profiling of DNA breaks in situ

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  • Site-wide: 10,428
  • In molecular biology: 283
• Year to date:
  • Site-wide: 76,254
• Since beginning of last month:
  • Site-wide: 118,169

Direct in vivo mapping of functional suppressors in glioblastoma genome

• Downloaded 1,571 times
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  • Site-wide: 11,016
  • In systems biology: 207
• Year to date:
  • Site-wide: 54,283
• Since beginning of last month:
  • Site-wide: 27,980

The CAFA challenge reports improved protein function prediction and new functional annotations for hundreds of genes through experimental screens

• Downloaded 1,551 times
• Download rankings, all-time:
  • Site-wide: 11,219
  • In bioinformatics: 1,363
• Year to date:
  • Site-wide: 34,234
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  • Site-wide: 44,082

Cas13b is a Type VI-B CRISPR-associated RNA-Guided RNase differentially regulated by accessory proteins Csx27 and Csx28

• Downloaded 1,549 times
• Download rankings, all-time:
  • Site-wide: 11,240
  • In microbiology: 634
• Year to date:
  • Site-wide: 119,323
• Since beginning of last month:
  • Site-wide: 133,146

Pooled AAV-CRISPR Screen with Targeted Amplicon Sequencing

• Downloaded 1,399 times
• Download rankings, all-time:
  • Site-wide: 13,090
  • In systems biology: 246
• Year to date:
  • Site-wide: 52,201
• Since beginning of last month:
  • Site-wide: 23,513

Human Pluripotent Stem Cell-Derived Neural Cells and Brain Organoids Reveal SARS-CoV-2 Neurotropism

• Downloaded 1,370 times
• Download rankings, all-time:
  • Site-wide: 13,525
  • In neuroscience: 1,556
• Year to date:
  • Site-wide: 13,445
• Since beginning of last month:
  • Site-wide: 11,034

GUIDES: sgRNA design for loss-of-function screens

• Downloaded 1,222 times
• Download rankings, all-time:
  • Site-wide: 16,060
  • In genetics: 778
• Year to date:
  • Site-wide: 93,695
• Since beginning of last month:
  • Site-wide: 77,037

Assessing the impact of a symptom-based mass screening and testing intervention during a novel infectious disease outbreak: The case of COVID-19

• Downloaded 985 times
• Download rankings, all-time:
  • Site-wide: 21,982
  • In infectious diseases: 1,797
• Year to date:
  • Site-wide: 56,654
• Since beginning of last month:
  • Site-wide: 57,157

Multi-cellular communities are perturbed in the aging human brain and with Alzheimer's disease

• Downloaded 823 times
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  • Site-wide: 28,462
  • In neuroscience: 3,791
• Year to date:
  • Site-wide: 2,470
• Since beginning of last month:
  • Site-wide: 7,272

Optimization of multiplexed CRISPR/Cas9 system for highly efficient genome editing in Setaria viridis

• Downloaded 726 times
• Download rankings, all-time:
  • Site-wide: 33,923
  • In plant biology: 664
• Year to date:
  • Site-wide: 24,729
• Since beginning of last month:
  • Site-wide: 16,429

The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterflies

• Downloaded 712 times
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  • Site-wide: 34,813
  • In evolutionary biology: 1,699
• Year to date:
  • Site-wide: 24,660
• Since beginning of last month:
  • Site-wide: 22,898

Essentiality of CREBBP in EP300 truncated B-cell lymphoma revealed by genome-wide CRISPR-Cas9 screen

• Downloaded 676 times
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  • Site-wide: 37,299
  • In cancer biology: 997
• Year to date:
  • Site-wide: 27,249
• Since beginning of last month:
  • Site-wide: 32,321

Novel gene-specific translation mechanism of dysregulated, chronic inflammation reveals promising, multifaceted COVID-19 therapeutics

• Downloaded 675 times
• Download rankings, all-time:
  • Site-wide: 37,429
  • In systems biology: 832
• Year to date:
  • Site-wide: 10,707
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  • Site-wide: None

An R Implementation of Tumor-Stroma-Immune Transcriptome Deconvolution Pipeline using DeMixT

• Downloaded 538 times
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  • Site-wide: 49,688
  • In bioinformatics: 5,146
• Year to date:
  • Site-wide: 82,687
• Since beginning of last month:
  • Site-wide: 116,986

A Genetically Encoded Trimethylsilyl 1D 1H-NMR Probe for Conformation Change in Large Membrane Protein Complexes

• Downloaded 455 times
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  • Site-wide: 60,028
  • In biochemistry: 1,608
• Year to date:
  • Site-wide: 87,342
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  • Site-wide: 85,233

PRH1 mediates ARF7-LBD dependent auxin signaling to regulate lateral root development in Arabidopsis thaliana

• Downloaded 455 times
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  • Site-wide: 60,048
  • In plant biology: 1,513
• Year to date:
  • Site-wide: 105,222
• Since beginning of last month:
  • Site-wide: 105,690

Movement behavioral plasticity of benthic diatoms driven by optimal foraging

• Downloaded 421 times
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  • Site-wide: 65,025
  • In ecology: 1,789
• Year to date:
  • Site-wide: 69,412
• Since beginning of last month:
  • Site-wide: 88,663

Conserved allosteric pathways for activation of TRPV3 revealed through engineering vanilloid-sensitivity

• Downloaded 354 times
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  • Site-wide: 76,564
  • In neuroscience: 11,679
• Year to date:
  • Site-wide: 85,690
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  • Site-wide: 60,452

Regulatory B cells contribute to the alleviation of colitis induced by Dextran Sulphate Sodium after H.pylori infection

• Downloaded 318 times
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  • Site-wide: 83,515
  • In immunology: 2,356
• Year to date:
  • Site-wide: 87,897
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  • Site-wide: 96,152

Effect of recombinant phycocyanin on photosynthetic system in Chlamydomonas reinhardtii

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  • Site-wide: 104,321
  • In molecular biology: 3,091
• Year to date:
  • Site-wide: 79,322
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  • Site-wide: 80,521

Photosynthetic resistance and resilience under drought and rewatering in maize plants

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  • Site-wide: 120,883
  • In plant biology: 3,382
• Year to date:
  • Site-wide: 87,364
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  • Site-wide: 83,533

Regulating AMPK and insulin level by Vinegar, Swimming and Refeeding on High-Fat Diet Rats to Rebuild Lipid Homeostasis

• Downloaded 140 times
• Download rankings, all-time:
  • Site-wide: 122,542
  • In molecular biology: 3,660
• Year to date:
  • Site-wide: 121,023
• Since beginning of last month:
  • Site-wide: 130,366

Evaluating the clinical performance of a novel dual-target stool DNA test for colorectal cancer detection

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  • Site-wide: 130,217
  • In gastroenterology: 169
• Year to date:
  • Site-wide: 32,289
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  • Site-wide: 11,140