Author: George Church

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Preprints

Vibrio natriegens, a new genomic powerhouse

• Downloaded 18,188 times
• Download rankings, all-time:
  • Site-wide: 356
  • In genomics: 17
• Year to date:
  • Site-wide: 2,887
• Since beginning of last month:
  • Site-wide: 2,207

Reversal of ageing- and injury-induced vision loss by Tet-dependent epigenetic reprogramming

• Downloaded 17,627 times
• Download rankings, all-time:
  • Site-wide: 365
  • In molecular biology: 4
• Year to date:
  • Site-wide: 759
• Since beginning of last month:
  • Site-wide: 694

Unified rational protein engineering with sequence-only deep representation learning

• Downloaded 10,773 times
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  • Site-wide: 679
  • In synthetic biology: 4
• Year to date:
  • Site-wide: 4,548
• Since beginning of last month:
  • Site-wide: None

Inter-homologue repair in fertilized human eggs?

• Downloaded 10,311 times
• Download rankings, all-time:
  • Site-wide: 723
  • In cell biology: 6
• Year to date:
  • Site-wide: 48,584
• Since beginning of last month:
  • Site-wide: 30,159

Expansion Sequencing: Spatially Precise In Situ Transcriptomics in Intact Biological Systems

• Downloaded 8,070 times
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  • Site-wide: 984
  • In genomics: 91
• Year to date:
  • Site-wide: 501
• Since beginning of last month:
  • Site-wide: 466

Conneconomics: The Economics of Dense, Large-Scale, High-Resolution Neural Connectomics

• Downloaded 6,532 times
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  • Site-wide: 1,279
  • In neuroscience: 62
• Year to date:
  • Site-wide: 19,502
• Since beginning of last month:
  • Site-wide: 26,738

Extensive sequencing of seven human genomes to characterize benchmark reference materials

• Downloaded 6,316 times
• Download rankings, all-time:
  • Site-wide: 1,339
  • In genomics: 129
• Year to date:
  • Site-wide: 33,118
• Since beginning of last month:
  • Site-wide: 35,039

Highly-efficient Cas9-mediated transcriptional programming

• Downloaded 6,216 times
• Download rankings, all-time:
  • Site-wide: 1,367
  • In synthetic biology: 11
• Year to date:
  • Site-wide: 8,502
• Since beginning of last month:
  • Site-wide: 5,999

Rapidly evolving homing CRISPR barcodes

• Downloaded 6,117 times
• Download rankings, all-time:
  • Site-wide: 1,394
  • In synthetic biology: 12
• Year to date:
  • Site-wide: 49,062
• Since beginning of last month:
  • Site-wide: 39,718

Enabling large-scale genome editing by reducing DNA nicking

• Downloaded 6,019 times
• Download rankings, all-time:
  • Site-wide: 1,431
  • In synthetic biology: 13
• Year to date:
  • Site-wide: 36,018
• Since beginning of last month:
  • Site-wide: None

Highly multiplexed in situ protein imaging with signal amplification by Immuno-SABER

• Downloaded 5,635 times
• Download rankings, all-time:
  • Site-wide: 1,593
  • In cell biology: 20
• Year to date:
  • Site-wide: 20,000
• Since beginning of last month:
  • Site-wide: 19,363

Barcoded oligonucleotides ligated on RNA amplified for multiplex and parallel in-situ analyses

• Downloaded 5,344 times
• Download rankings, all-time:
  • Site-wide: 1,703
  • In cell biology: 24
• Year to date:
  • Site-wide: 6,377
• Since beginning of last month:
  • Site-wide: 5,041

Daisy-chain gene drives for the alteration of local populations

• Downloaded 5,319 times
• Download rankings, all-time:
  • Site-wide: 1,712
  • In synthetic biology: 14
• Year to date:
  • Site-wide: 82,689
• Since beginning of last month:
  • Site-wide: None

The predictive power of the microbiome exceeds that of genome-wide association studies in the discrimination of complex human disease

• Downloaded 5,091 times
• Download rankings, all-time:
  • Site-wide: 1,801
  • In bioinformatics: 153
• Year to date:
  • Site-wide: 6,620
• Since beginning of last month:
  • Site-wide: 5,250

CasFinder: Flexible algorithm for identifying specific Cas9 targets in genomes

• Downloaded 5,083 times
• Download rankings, all-time:
  • Site-wide: 1,804
  • In genomics: 185
• Year to date:
  • Site-wide: 23,581
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  • Site-wide: None

Enzymatic DNA synthesis for digital information storage

• Downloaded 5,035 times
• Download rankings, all-time:
  • Site-wide: 1,834
  • In synthetic biology: 16
• Year to date:
  • Site-wide: 18,970
• Since beginning of last month:
  • Site-wide: 14,903

Current CRISPR gene drive systems are likely to be highly invasive in wild populations

• Downloaded 4,024 times
• Download rankings, all-time:
  • Site-wide: 2,503
  • In synthetic biology: 23
• Year to date:
  • Site-wide: 94,674
• Since beginning of last month:
  • Site-wide: None

Accurate chromosome-scale haplotype-resolved assembly of human genomes

• Downloaded 3,780 times
• Download rankings, all-time:
  • Site-wide: 2,759
  • In bioinformatics: 267
• Year to date:
  • Site-wide: 4,618
• Since beginning of last month:
  • Site-wide: 6,550

Extensive Mammalian Germline Genome Engineering

• Downloaded 3,411 times
• Download rankings, all-time:
  • Site-wide: 3,217
  • In synthetic biology: 30
• Year to date:
  • Site-wide: 19,193
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  • Site-wide: None

A homing CRISPR mouse resource for barcoding and lineage tracing

• Downloaded 3,272 times
• Download rankings, all-time:
  • Site-wide: 3,416
  • In synthetic biology: 32
• Year to date:
  • Site-wide: 103,828
• Since beginning of last month:
  • Site-wide: None

Rapid Sequential In Situ Multiplexing With DNA-Exchange-Imaging

• Downloaded 3,187 times
• Download rankings, all-time:
  • Site-wide: 3,548
  • In bioengineering: 35
• Year to date:
  • Site-wide: 84,161
• Since beginning of last month:
  • Site-wide: None

Evolutionary dynamics of CRISPR gene drives

• Downloaded 2,715 times
• Download rankings, all-time:
  • Site-wide: 4,427
  • In synthetic biology: 42
• Year to date:
  • Site-wide: 71,955
• Since beginning of last month:
  • Site-wide: None

Recombineering in Vibrio natriegens

• Downloaded 2,695 times
• Download rankings, all-time:
  • Site-wide: 4,482
  • In synthetic biology: 43
• Year to date:
  • Site-wide: 18,975
• Since beginning of last month:
  • Site-wide: 25,705

Assembly of Radically Recoded E. coli Genome Segments

• Downloaded 2,652 times
• Download rankings, all-time:
  • Site-wide: 4,606
  • In synthetic biology: 46
• Year to date:
  • Site-wide: 76,766
• Since beginning of last month:
  • Site-wide: None

Multiplexed in situ protein imaging using DNA-barcoded antibodies with extended hybridization chain reactions

• Downloaded 2,621 times
• Download rankings, all-time:
  • Site-wide: 4,696
  • In cell biology: 90
• Year to date:
  • Site-wide: 5,838
• Since beginning of last month:
  • Site-wide: 4,205

Precise Cas9 targeting enables genomic mutation prevention

• Downloaded 2,588 times
• Download rankings, all-time:
  • Site-wide: 4,763
  • In synthetic biology: 50
• Year to date:
  • Site-wide: 67,509
• Since beginning of last month:
  • Site-wide: 62,528

Rapid and scalable in vitro production of single-stranded DNA

• Downloaded 2,488 times
• Download rankings, all-time:
  • Site-wide: 5,061
  • In molecular biology: 111
• Year to date:
  • Site-wide: 36,047
• Since beginning of last month:
  • Site-wide: 29,704

The experimental design and data interpretation in ‘Unexpected mutations after CRISPR Cas9 editing in vivo’ by Schaefer et al. are insufficient to support the conclusions drawn by the authors

• Downloaded 2,219 times
• Download rankings, all-time:
  • Site-wide: 5,980
  • In genetics: 288
• Year to date:
  • Site-wide: 43,692
• Since beginning of last month:
  • Site-wide: 47,583

Efficient in situ barcode sequencing using padlock probe-based BaristaSeq

• Downloaded 1,950 times
• Download rankings, all-time:
  • Site-wide: 7,211
  • In molecular biology: 176
• Year to date:
  • Site-wide: 41,938
• Since beginning of last month:
  • Site-wide: None

Genetic design automation for autonomous formation of multicellular shapes from a single cell progenitor

• Downloaded 1,771 times
• Download rankings, all-time:
  • Site-wide: 8,338
  • In synthetic biology: 88
• Year to date:
  • Site-wide: 17,860
• Since beginning of last month:
  • Site-wide: None

High-throughput creation and functional profiling of eukaryotic DNA sequence variant libraries using CRISPR/Cas9

• Downloaded 1,770 times
• Download rankings, all-time:
  • Site-wide: 8,346
  • In bioengineering: 136
• Year to date:
  • Site-wide: 71,580
• Since beginning of last month:
  • Site-wide: 59,370

An orthogonal differentiation platform for genomically programming stem cells, organoids, and bioprinted tissues

• Downloaded 1,741 times
• Download rankings, all-time:
  • Site-wide: 8,567
  • In bioengineering: 140
• Year to date:
  • Site-wide: 10,244
• Since beginning of last month:
  • Site-wide: None

The whale shark genome reveals how genomic and physiological properties scale with body size

• Downloaded 1,708 times
• Download rankings, all-time:
  • Site-wide: 8,834
  • In genomics: 994
• Year to date:
  • Site-wide: 77,190
• Since beginning of last month:
  • Site-wide: 73,877

A systematic comparison of error correction enzymes by next-generation sequencing

• Downloaded 1,646 times
• Download rankings, all-time:
  • Site-wide: 9,341
  • In synthetic biology: 97
• Year to date:
  • Site-wide: 36,395
• Since beginning of last month:
  • Site-wide: 27,107

Photon-directed Multiplexed Enzymatic DNA Synthesis for Molecular Digital Data Storage

• Downloaded 1,576 times
• Download rankings, all-time:
  • Site-wide: 9,965
  • In synthetic biology: 106
• Year to date:
  • Site-wide: 26,540
• Since beginning of last month:
  • Site-wide: None

A DNA nanoscope that identifies and precisely localizes over a hundred unique molecular features with nanometer accuracy

• Downloaded 1,563 times
• Download rankings, all-time:
  • Site-wide: 10,072
  • In biochemistry: 195
• Year to date:
  • Site-wide: 5,820
• Since beginning of last month:
  • Site-wide: 4,201

Improved bacterial recombineering by parallelized protein discovery

• Downloaded 1,431 times
• Download rankings, all-time:
  • Site-wide: 11,468
  • In microbiology: 640
• Year to date:
  • Site-wide: 35,577
• Since beginning of last month:
  • Site-wide: 40,788

Reprogramming protein kinase substrate specificity through synthetic mutations

• Downloaded 1,427 times
• Download rankings, all-time:
  • Site-wide: 11,511
  • In biochemistry: 219
• Year to date:
  • Site-wide: 69,961
• Since beginning of last month:
  • Site-wide: None

Induced Pluripotent Stem Cell-derived CAR-Macrophage Cells with Antigen-dependent Anti-Cancer Cell Functions for Liquid and Solid Tumors

• Downloaded 1,413 times
• Download rankings, all-time:
  • Site-wide: 11,664
  • In cell biology: 327
• Year to date:
  • Site-wide: 5,101
• Since beginning of last month:
  • Site-wide: 5,339

Concerning RNA-Guided Gene Drives for the Alteration of Wild Populations

• Downloaded 1,411 times
• Download rankings, all-time:
  • Site-wide: 11,708
  • In synthetic biology: 130
• Year to date:
  • Site-wide: 84,624
• Since beginning of last month:
  • Site-wide: 93,251

Multi-pronged human protein mimicry by SARS-CoV-2 reveals bifurcating potential for MHC detection and immune evasion

• Downloaded 1,405 times
• Download rankings, all-time:
  • Site-wide: 11,783
  • In evolutionary biology: 419
• Year to date:
  • Site-wide: 4,822
• Since beginning of last month:
  • Site-wide: None

CRISPR-mediated biocontainment

• Downloaded 1,381 times
• Download rankings, all-time:
  • Site-wide: 12,117
  • In synthetic biology: 139
• Year to date:
  • Site-wide: 33,564
• Since beginning of last month:
  • Site-wide: 23,916

Enhanced bacterial immunity and mammalian genome editing via RNA polymerase-mediated dislodging of Cas9 from double strand DNA breaks.

• Downloaded 1,361 times
• Download rankings, all-time:
  • Site-wide: 12,363
  • In molecular biology: 340
• Year to date:
  • Site-wide: 88,545
• Since beginning of last month:
  • Site-wide: None

Engineering post-translational proofreading to discriminate non-standard amino acids

• Downloaded 1,282 times
• Download rankings, all-time:
  • Site-wide: 13,533
  • In synthetic biology: 160
• Year to date:
  • Site-wide: 67,521
• Since beginning of last month:
  • Site-wide: 47,097

Systemic post-translational control of bacterial metabolism regulates adaptation in dynamic environments

• Downloaded 1,242 times
• Download rankings, all-time:
  • Site-wide: 14,175
  • In systems biology: 286
• Year to date:
  • Site-wide: 30,581
• Since beginning of last month:
  • Site-wide: 17,255

Optimizing complex phenotypes through model-guided multiplex genome engineering

• Downloaded 1,201 times
• Download rankings, all-time:
  • Site-wide: 14,931
  • In synthetic biology: 185
• Year to date:
  • Site-wide: 83,361
• Since beginning of last month:
  • Site-wide: None

Fluorescent in situ sequencing of DNA barcoded antibodies

• Downloaded 1,096 times
• Download rankings, all-time:
  • Site-wide: 17,165
  • In neuroscience: 2,156
• Year to date:
  • Site-wide: 8,625
• Since beginning of last month:
  • Site-wide: 5,530

Biosensor libraries harness large classes of binding domains for allosteric transcription regulators

• Downloaded 1,094 times
• Download rankings, all-time:
  • Site-wide: 17,210
  • In synthetic biology: 214
• Year to date:
  • Site-wide: 101,951
• Since beginning of last month:
  • Site-wide: 73,212

Massively parallel quantification of CRISPR editing in cells by TRAP-seq enables better design of Cas9, ABE, CBE gRNAs of high efficiency and accuracy

• Downloaded 1,030 times
• Download rankings, all-time:
  • Site-wide: 18,836
  • In systems biology: 391
• Year to date:
  • Site-wide: 23,765
• Since beginning of last month:
  • Site-wide: 33,266

Characterizing the portability of RecT-mediated oligonucleotide recombination

• Downloaded 1,017 times
• Download rankings, all-time:
  • Site-wide: 19,169
  • In synthetic biology: 242
• Year to date:
  • Site-wide: 16,647
• Since beginning of last month:
  • Site-wide: 15,320

Significant abundance of cis configurations of mutations in diploid human genomes

• Downloaded 1,013 times
• Download rankings, all-time:
  • Site-wide: 19,310
  • In genomics: 1,979
• Year to date:
  • Site-wide: 35,880
• Since beginning of last month:
  • Site-wide: 33,740

Citizen-Centered, Auditable, and Privacy-Preserving Population Genomics

• Downloaded 976 times
• Download rankings, all-time:
  • Site-wide: 20,315
  • In genomics: 2,052
• Year to date:
  • Site-wide: 18,736
• Since beginning of last month:
  • Site-wide: 19,122

Dissecting limiting factors of the Protein synthesis Using Recombinant Elements (PURE) system

• Downloaded 956 times
• Download rankings, all-time:
  • Site-wide: 20,941
  • In bioengineering: 352
• Year to date:
  • Site-wide: 118,193
• Since beginning of last month:
  • Site-wide: None

Attribution of genetic engineering: a practical and accurate machine learning toolkit for biosecurity

• Downloaded 947 times
• Download rankings, all-time:
  • Site-wide: 21,236
  • In synthetic biology: 270
• Year to date:
  • Site-wide: 31,246
• Since beginning of last month:
  • Site-wide: 27,317

Long-term adaptive evolution of genomically recoded Escherichia coli

• Downloaded 875 times
• Download rankings, all-time:
  • Site-wide: 23,801
  • In evolutionary biology: 1,114
• Year to date:
  • Site-wide: 96,694
• Since beginning of last month:
  • Site-wide: 72,791

Ethnically relevant consensus Korean reference genome towards personal reference genomes

• Downloaded 854 times
• Download rankings, all-time:
  • Site-wide: 24,634
  • In genomics: 2,375
• Year to date:
  • Site-wide: 77,909
• Since beginning of last month:
  • Site-wide: 75,332

Toward D-Peptide Biosynthesis: Elongation Factor P Enables Ribosomal Incorporation Of Consecutive D-Amino Acids

• Downloaded 791 times
• Download rankings, all-time:
  • Site-wide: 27,369
  • In biochemistry: 649
• Year to date:
  • Site-wide: 66,563
• Since beginning of last month:
  • Site-wide: 57,716

Towards Practical and Robust DNA-based Data Archiving by Codec System Named ‘Yin-Yang’

• Downloaded 764 times
• Download rankings, all-time:
  • Site-wide: 28,718
  • In synthetic biology: 369
• Year to date:
  • Site-wide: 30,309
• Since beginning of last month:
  • Site-wide: 20,829

Spatial Information in Large-Scale Neural Recordings

• Downloaded 759 times
• Download rankings, all-time:
  • Site-wide: 29,013
  • In neuroscience: 3,969
• Year to date:
  • Site-wide: 102,689
• Since beginning of last month:
  • Site-wide: 77,926

Cushing's Syndrome mutant PKAL205R exhibits altered substrate specificity

• Downloaded 729 times
• Download rankings, all-time:
  • Site-wide: 30,711
  • In biochemistry: 737
• Year to date:
  • Site-wide: 104,438
• Since beginning of last month:
  • Site-wide: 57,593

Robust differentiation of human pluripotent stem cells into endothelial cells via temporal modulation of ETV2 with modified mRNA

• Downloaded 670 times
• Download rankings, all-time:
  • Site-wide: 34,453
  • In cell biology: 1,383
• Year to date:
  • Site-wide: 44,424
• Since beginning of last month:
  • Site-wide: 36,670

L1CAM is not Associated with Extracellular Vesicles in Human Cerebrospinal Fluid or Plasma

• Downloaded 652 times
• Download rankings, all-time:
  • Site-wide: 35,744
  • In neuroscience: 5,035
• Year to date:
  • Site-wide: 7,568
• Since beginning of last month:
  • Site-wide: 10,011

Emergent rules for codon choice elucidated by editing rare arginine codons in Escherichia coli

• Downloaded 568 times
• Download rankings, all-time:
  • Site-wide: 42,549
  • In genomics: 3,495
• Year to date:
  • Site-wide: 115,928
• Since beginning of last month:
  • Site-wide: None

Simulating serial-target antibacterial drug synergies using flux balance analysis

• Downloaded 514 times
• Download rankings, all-time:
  • Site-wide: 48,028
  • In systems biology: 1,126
• Year to date:
  • Site-wide: 84,343
• Since beginning of last month:
  • Site-wide: 59,141

Multiplex single-molecule kinetics of nanopore-coupled polymerases

• Downloaded 499 times
• Download rankings, all-time:
  • Site-wide: 49,689
  • In synthetic biology: 601
• Year to date:
  • Site-wide: 34,173
• Since beginning of last month:
  • Site-wide: 43,589

Physiological Assembly Of Functionally Active 30S Ribosomal Subunits From In Vitro Synthesized Parts

• Downloaded 447 times
• Download rankings, all-time:
  • Site-wide: 56,167
  • In biochemistry: 1,481
• Year to date:
  • Site-wide: 108,244
• Since beginning of last month:
  • Site-wide: None

TBDB: A database of structurally annotated T-box riboswitch-tRNA pairs

• Downloaded 424 times
• Download rankings, all-time:
  • Site-wide: 59,281
  • In bioinformatics: 5,916
• Year to date:
  • Site-wide: 38,998
• Since beginning of last month:
  • Site-wide: 28,984

Comparison of Extracellular Vesicle Isolation Methods from Human Biofluids using Single Molecule Arrays

• Downloaded 301 times
• Download rankings, all-time:
  • Site-wide: 80,119
  • In biochemistry: 2,302
• Year to date:
  • Site-wide: 12,119
• Since beginning of last month:
  • Site-wide: 12,483